The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric area under curve method for estimation of tramadol hydrochloride. This Area under Curve of UV spectrum between 266 to 276 nm was validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 30-150 μg/ml. The value of correlation coefficient was 1. Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (97.94% to 106.98%) showed accuracy of the method. LOD and LOQ were calculated as 0.8937 μg/ml and 2.70 μg/ml respectively. The developed method can be used for routine estimation of tramadol hydrochloride in bulk and capsule dosage forms.
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